Comment on "The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles".
نویسندگان
چکیده
Zhang et al. (Research Articles, 13 March 2009, p. 1448) reported that synaptic vesicles usually release neurotransmitter through a kiss-and-run mechanism occurring within 1 second but that full collapse of the vesicles becomes more prevalent with repeated stimuli. We report that the kinetics of vesicle retrieval do not change during a stimulus train, with endocytosis occurring in 10 to 15 seconds.
منابع مشابه
The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles.
Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single q...
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Granseth et al. argue that vesicle retrieval at hippocampal synapses is fully accounted for by a single mode of endocytosis. However, their assay focused on readily releasable pool vesicles (RRP), not RRP + reserve pool vesicles in tandem, and therefore cannot detect pool-dependent changes in vesicle-retrieval kinetics during a stimulus train. Using a probe similar to theirs, we observed rapid ...
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The kinetics of exo-endocytotic recycling could restrict information transfer at central synapses if neurotransmission were entirely reliant on classical full-collapse fusion. Nonclassical fusion retrieval by kiss-and-run would be kinetically advantageous but remains controversial. We used a hydrophilic quencher, bromophenol blue (BPB), to help detect nonclassical events. Upon stimulation, extr...
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ورودعنوان ژورنال:
- Science
دوره 325 5947 شماره
صفحات -
تاریخ انتشار 2009